Determination of inositol 1,4,5-trisphosphate levels in Dictyostelium by isotope dilution assay
نویسندگان
چکیده
منابع مشابه
Cyclic AMP stimulates accumulation of inositol trisphosphate in Dictyostelium.
Previous studies indicated that in Dictyostelium amoebae signal transmission from cell surface cyclic AMP receptors to intracellular events concerned with chemotaxis involves inositol 1,4,5-trisphosphate (1,4,5-IP3): micromolar amounts of 1,4,5-IP3 or Ca2+ were found to mimic the effects of chemoattractants and 1,4,5-IP3 triggered release of Ca2+ from non-mitochondrial stores. Here we report a ...
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A fast, easy-to-handle and cost-effective analytical method for 11 mycotoxins currently regulated in maize and other cereal-based food products in Europe was developed and validated for maize. The method is based on two extraction steps using different acidified acetonitrile–water mixtures. Separation is achieved using ultrahigh-performance liquid chromatography (UHPLC) by a linear water–methan...
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Permeabilization of amoebae of Dictyostelium discoideum with saponin was found not to uncouple the chemotactic cell surface cyclic AMP receptors from inositol trisphosphate (IP3) formation, and stimulation of permeabilized amoebae with 50 nM-cyclic AMP produced peaks of IP3 at 5, 15 and 30 s in a manner comparable to that seen previously in non-permeabilized cells. The possible involvement of a...
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BETH BURNSIDE, CHRIS KOZAK, and F. C. KAFATOS. From the Marine Biological Laboratory, Woods Hole, Massachusetts 02543 . Dr . Burnside's present address is the Department of Anatomy, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104 . Dr . Kozak's present address is the Department of Biology, Yale University, New Haven, Connecticut 06520 . Dr. Kafatos' present addr...
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 1989
ISSN: 0003-2697
DOI: 10.1016/0003-2697(89)90024-9